| 64 | Mikos, Sylwia (UCW) def. Grace Gardner (TU-W) 6-0, 6-0 |
| 64 | Kanthaphixay, Alaina (NCC) def. Anna Winslow (KENW) 6-3, 6-1 |
| 64 | Ashley Spirrison (TRINEW) def. Sydney Khosla (WITW) 6-0, 6-0 |
| 64 | Sophia Huynh (WASHU) def. Gabby Quintero (PRINCIP) 6-0, 6-0 |
| 64 | Savannah Bright (CWR-W) def. Otilia Oita (WOOW) 4-6, 6-2, 10-4 |
| 64 | Megan Reese (JCUW) def. Oda, Lauren (AUGIEW) 6-2, 6-2 |
| 64 | Sarah Robertson (DENW) def. Lucy Chamberlin (SMC) 6-0, 6-0 |
| 64 | Juliana Johnson (ADRIAN) def. Katherine Price (DPUW) 7-5, 7-5 |
| 64 | Hannah Kassaie (CWR-W) def. Mireia Birosta (GREEN) 6-1, 6-0 |
| 64 | Divya Sharma (WASHU) def. Obradovic, Nikol (MILLIKIN) 6-3, 7-5 |
| 64 | Maicy McCarty (HOPE) def. Caroline Lopez (DENW) 6-4, 6-2 |
| 64 | Lillia Walter (OTT) def. Kathryn Valade (ALBION) 6-0, 6-0 |
| 64 | Park, Lauren (UCW) def. Hougland, Hannah (NCC) 6-1, 6-3 |
| 64 | Ashtyn Morris (BW-W) def. Ellen Gardner (DPUW) 6-3, 7-6 (8-6) |
| 64 | Daria Beshentseva (KENW) def. Schoeck, Lily (AUGIEW) 6-1, 6-1 |
| 64 | Margaret Bugnacki (LFC) def. Caroline Norton (KZOO-W) 7-6 (7-5), 6-3 |
| 64 | Hannah Johnston (WASHU) def. Erin Brezovar (WEB) 6-3, 6-2 |
| 64 | Yuan, Miranda (UCW) def. Sydney Jackson (HOPE) 7-6 (7-5), 6-4 |
| 64 | Maria Jose Campos (LFC) def. Ella Knight (KZOO-W) 6-1, 6-2 |
| 64 | Madeleine Eiss (CWR-W) def. Ashley Isaac (WITW) 6-3, 7-5 |
| 64 | Erin Storrer (OTT) def. Addison Brannon (GREEN) 6-0, 6-0 |
| 64 | Smashey, Kaeli (NCC) def. Kathleen McLeod (SMC) 6-2, 6-1 |
| 64 | Markus, Emily (MILLIKIN) def. Katherine Taylor (TU-W) 4-6, 6-3, 11-9 |
| 64 | Sydney Cianciola (DENW) def. Eleni Dakos (KENW) 6-3, 7-6 (7-5) |
| 64 | Monique Brual (DENW) def. Sullivan Patel (HOPE) 6-4, 6-3 |
| 64 | Victoria Vasquez (KENW) def. Saige Jost (ALBION) 6-0, 6-0 |
| 64 | Gabriela Heideman (BW-W) def. Maddie Hurley (KZOO-W) 6-3, 7-6 (7-3) |
| 64 | Randi Hendricks (WASHU) def. Jadyn Davis (TRINEW) 6-0, 6-0 |
| 64 | Kat Wilder (DPUW) def. Ellie Himebauch (ADRIAN) 6-2, 6-2 |
| 64 | Camila Gomez (PRINCIP) def. Cherry Patlolla (WOOW) 6-1, 6-4 |
| 64 | Anna Kan (CWR-W) def. Hanulikova, Caitlyn (AUGIEW) 6-4, 0-6, 10-4 |
| 64 | Guo, Shianna (UCW) def. Mia Zivkovic (JCUW) 6-3, 6-2 |
| 32 | Mikos, Sylwia (UCW) def. Kanthaphixay, Alaina (NCC) 6-3, 6-1 |
| 32 | Ashley Spirrison (TRINEW) def. Sophia Huynh (WASHU) 6-1, 3-6, 10-8 |
| 32 | Savannah Bright (CWR-W) def. Megan Reese (JCUW) 7-5, 6-3 |
| 32 | Sarah Robertson (DENW) def. Juliana Johnson (ADRIAN) 3-6, 6-1, 10-3 |
| 32 | Hannah Kassaie (CWR-W) def. Divya Sharma (WASHU) 6-0, 6-3 |
| 32 | Maicy McCarty (HOPE) def. Lillia Walter (OTT) 6-3, 6-0 |
| 32 | Park, Lauren (UCW) def. Ashtyn Morris (BW-W) 6-1, 6-1 |
| 32 | Daria Beshentseva (KENW) def. Margaret Bugnacki (LFC) 6-3, 6-2 |
| 32 | Yuan, Miranda (UCW) def. Hannah Johnston (WASHU) 6-3, 6-1 |
| 32 | Madeleine Eiss (CWR-W) def. Maria Jose Campos (LFC) 6-0, 6-0 |
| 32 | Erin Storrer (OTT) def. Smashey, Kaeli (NCC) 7-5, 6-4 |
| 32 | Sydney Cianciola (DENW) def. Markus, Emily (MILLIKIN) 6-2, 3-6, 10-7 |
| 32 | Victoria Vasquez (KENW) def. Monique Brual (DENW) 6-0, 6-1 |
| 32 | Randi Hendricks (WASHU) def. Gabriela Heideman (BW-W) 6-1, 6-1 |
| 32 | Camila Gomez (PRINCIP) def. Kat Wilder (DPUW) 6-2, 6-1 |
| 32 | Guo, Shianna (UCW) def. Anna Kan (CWR-W) 7-5, 6-0 |
| 16 | Mikos, Sylwia (UCW) def. Ashley Spirrison (TRINEW) 6-0, 6-0 |
| 16 | Sarah Robertson (DENW) def. Savannah Bright (CWR-W) 6-6, 1-0 |
| 16 | Hannah Kassaie (CWR-W) def. Maicy McCarty (HOPE) 6-4, 6-3 |
| 16 | Park, Lauren (UCW) def. Daria Beshentseva (KENW) 6-0, 6-2 |
| 16 | Yuan, Miranda (UCW) def. Madeleine Eiss (CWR-W) 6-1, 6-0 |
| 16 | Erin Storrer (OTT) def. Sydney Cianciola (DENW) 4-6, 6-4, 6-2 |
| 16 | Victoria Vasquez (KENW) def. Randi Hendricks (WASHU) 6-3, 7-5 |
| 16 | Guo, Shianna (UCW) def. Camila Gomez (PRINCIP) 6-3, 6-1 |
| QF | Mikos, Sylwia (UCW) def. Sarah Robertson (DENW) 6-0, 6-0 |
| QF | Hannah Kassaie (CWR-W) def. Park, Lauren (UCW) 6-2, 6-2 |
| QF | Yuan, Miranda (UCW) def. Erin Storrer (OTT) 6-0, 5-7, 6-3 |
| QF | Victoria Vasquez (KENW) def. Guo, Shianna (UCW) 4-6, 7-6 (7-3), 6-2 |
| SF | Mikos, Sylwia (UCW) def. Hannah Kassaie (CWR-W) 6-2, 6-3 |
| SF | Yuan, Miranda (UCW) def. Victoria Vasquez (KENW) 6-3, 3-6, 6-4 |
| F | Mikos, Sylwia (UCW) def. Yuan, Miranda (UCW) 6-2, 6-1 |
| C | Anna Winslow (KENW) def. Grace Gardner (TU-W) 6-3, 6-4 |
| C | Sydney Khosla (WITW) def. Gabby Quintero (PRINCIP) 6-0, 6-3 |
| C | Otilia Oita (WOOW) def. Oda, Lauren (AUGIEW) 6-1, 6-4 |
| C | Katherine Price (DPUW) def. Lucy Chamberlin (SMC) 6-3, 6-1 |
| C | Obradovic, Nikol (MILLIKIN) def. Mireia Birosta (GREEN) 5-7, 7-5, 10-8 |
| C | Caroline Lopez (DENW) def. Kathryn Valade (ALBION) 6-0, 6-0 |
| C | Hougland, Hannah (NCC) def. Ellen Gardner (DPUW) 2-6, 6-4, 12-10 |
| C | Schoeck, Lily (AUGIEW) def. Caroline Norton (KZOO-W) 5-0, retired |
| C | Sydney Jackson (HOPE) def. Erin Brezovar (WEB) 6-1, 6-2 |
| C | Ashley Isaac (WITW) def. Ella Knight (KZOO-W) 6-1, 6-0 |
| C | Kathleen McLeod (SMC) def. Addison Brannon (GREEN) 6-2, 0-6, 10-1 |
| C | Eleni Dakos (KENW) def. Katherine Taylor (TU-W) 6-3, 6-3 |
| C | Sullivan Patel (HOPE) def. Saige Jost (ALBION) 6-0, 6-1 |
| C | Maddie Hurley (KZOO-W) def. Jadyn Davis (TRINEW) 6-1, 6-3 |
| C | Ellie Himebauch (ADRIAN) def. Cherry Patlolla (WOOW) walkover |
| C | Mia Zivkovic (JCUW) def. Hanulikova, Caitlyn (AUGIEW) 6-0, 6-1 |
| C | Anna Winslow (KENW) def. Sydney Khosla (WITW) 6-2, 6-1 |
| C | Katherine Price (DPUW) def. Otilia Oita (WOOW) 6-3, 6-3 |
| C | Obradovic, Nikol (MILLIKIN) def. Caroline Lopez (DENW) 0-6, 7-5, 10-8 |
| C | Hougland, Hannah (NCC) def. Schoeck, Lily (AUGIEW) 6-3, 6-2 |
| C | Ashley Isaac (WITW) def. Sydney Jackson (HOPE) 3-6, 7-5, 10-6 |
| C | Eleni Dakos (KENW) def. Kathleen McLeod (SMC) 6-0, 6-1 |
| C | Sullivan Patel (HOPE) def. Maddie Hurley (KZOO-W) 6-1, 6-4 |
| C | Mia Zivkovic (JCUW) def. Ellie Himebauch (ADRIAN) 5-7, 6-1, 10-0 |
| CQ | Anna Winslow (KENW) def. Katherine Price (DPUW) 6-3, 6-2 |
| CQ | Obradovic, Nikol (MILLIKIN) def. Hougland, Hannah (NCC) 6-0, 6-3 |
| CQ | Ashley Isaac (WITW) def. Eleni Dakos (KENW) 6-4, 6-1 |
| CQ | Sullivan Patel (HOPE) def. Mia Zivkovic (JCUW) 6-1, 6-0 |
| CS | Anna Winslow (KENW) def. Obradovic, Nikol (MILLIKIN) 6-0, 6-1 |
| CS | Ashley Isaac (WITW) def. Sullivan Patel (HOPE) walkover |
| CF | Ashley Isaac (WITW) def. Anna Winslow (KENW) 6-1, 6-4 |
