| 64 | Erika Oku (KENW) def. Lily Cameron (DPUW) 6-2, 6-0 |
| 64 | Wang, Perene (UCW) def. Kanthaphixay, Alaina (NCC) 6-2, 6-1 |
| 64 | Julie Lord (LFC) def. Katherine Marks (ALLW) 7-6 (7-1), 6-1 |
| 64 | Savannah Bright (CWR-W) def. Monique Brual (DENW) 7-6 (7-3), 6-2 |
| 64 | Hannah Johnston (WASHUW) def. Qingyue Li (WOOW) 6-0, 7-5 |
| 64 | Hannah Cianciola (DENW) def. Barconi,Brianna (MILLIKIN) 6-0, 6-1 |
| 64 | Leah Newhof (HOPE) def. Jayde Robinson (BW-W19) 6-1, 6-3 |
| 64 | Caroline Norton (KZOO-W) def. Gonzaga, Ysabel (ELMHURST) 6-0, 6-1 |
| 64 | Amanda Bandrowski (HOPE) def. Megan Collins (KENW) 6-4, 6-2 |
| 64 | Emma Love (DPUW) def. Viuhkonen, Lisa (NPU) 6-2, 6-0 |
| 64 | Sydney Cianciola (DENW) def. Maddy Winarski (KZOO-W) 6-2, 6-0 |
| 64 | Zoe Park (WASHUW) def. Tebatso Duba (LFC) 6-2, 6-0 |
| 64 | Semenov, Nicole (UCW) def. Magee, Michaela (AUGIE) 6-0, 6-0 |
| 64 | Makayla Sutter (OTT) def. Stechow, Claire (WITT) 6-2, 6-3 |
| 64 | Ally Persky (WASHUW) def. Kyra Braun (TRINE-W) 6-0, 6-1 |
| 64 | Kayla Pukys (KENW) def. Nicole Cardwell (CWR-W) 2-6, 7-5, 6-2 |
| 64 | Timm, Annie (AUGIE) def. Hougland, Hannah (NCC) 1-6, 6-4, 6-2 |
| 64 | Trisha Sundaram (CWR-W) def. Cami Jones (HC-W) 6-1, 6-1 |
| 64 | Ng, Claudia (UCW) def. Bettner,Morgan (MILLIKIN) 6-0, 6-2 |
| 64 | Daria Beshentseva (KENW) def. Meyers, Paige (WITT) 6-1, 6-1 |
| 64 | Ilana Blackwood (KENW) def. Sanjna Tripathy (WASHUW) 7-5, 6-4 |
| 64 | Park, Lauren (UCW) def. Alex Cash (DENW) 6-1, 6-2 |
| 64 | Amanda Showalter (DPUW) def. Sydney Bergman (OTT) 6-3, 6-1 |
| 64 | Claire Hallock (HOPE) def. Ashtyn Morris (BW-W19) 6-1, 6-0 |
| 64 | Laura Hanselman (KZOO-W) def. Leeza Bodnar (LFC) 6-3, 6-4 |
| 64 | Sydney Jackson (HOPE) def. Gaby DeLeon (ALLW) 6-3, 6-2 |
| 64 | Leslie Ligier (WASHUW) def. Schoeck, Lily (AUGIE) 7-5, 6-0 |
| 64 | Anna Winslow (KENW) def. Ashley Spirrison (TRINE-W) 6-2, 6-1 |
| 64 | Erin Friedrich (DPUW) def. Pasquini, Nina (ELMHURST) 6-0, 6-0 |
| 64 | Xu, Catherine (UCW) def. Ishika Gupta (WOOW) 6-1, 6-0 |
| 64 | Divya Sharma (WASHUW) def. Viuhkonen, Linn (NPU) 6-0, 6-0 |
| 64 | Madeleine Paolucci (CWR-W) def. Sarah Robertson (DENW) 6-4, 6-1 |
| 32 | Erika Oku (KENW) def. Wang, Perene (UCW) 5-7, 6-3, 6-2 |
| 32 | Savannah Bright (CWR-W) def. Julie Lord (LFC) 7-6 (7-6), 6-1 |
| 32 | Hannah Johnston (WASHUW) def. Hannah Cianciola (DENW) 2-6, 6-4, 6-2 |
| 32 | Leah Newhof (HOPE) def. Caroline Norton (KZOO-W) 6-4, 6-4 |
| 32 | Amanda Bandrowski (HOPE) def. Emma Love (DPUW) 6-2, 6-2 |
| 32 | Sydney Cianciola (DENW) def. Zoe Park (WASHUW) 2-1, retired |
| 32 | Semenov, Nicole (UCW) def. Makayla Sutter (OTT) 6-0, 6-1 |
| 32 | Ally Persky (WASHUW) def. Kayla Pukys (KENW) 6-2, 6-2 |
| 32 | Trisha Sundaram (CWR-W) def. Timm, Annie (AUGIE) 6-0, 6-1 |
| 32 | Daria Beshentseva (KENW) def. Ng, Claudia (UCW) 3-6, 6-3, 6-3 |
| 32 | Park, Lauren (UCW) def. Ilana Blackwood (KENW) 6-3, 6-4 |
| 32 | Claire Hallock (HOPE) def. Amanda Showalter (DPUW) 6-2, 6-1 |
| 32 | Sydney Jackson (HOPE) def. Laura Hanselman (KZOO-W) 6-0, 6-3 |
| 32 | Leslie Ligier (WASHUW) def. Anna Winslow (KENW) 7-5, 5-7, 7-6 (7-2) |
| 32 | Xu, Catherine (UCW) def. Erin Friedrich (DPUW) 6-1, 6-0 |
| 32 | Divya Sharma (WASHUW) def. Madeleine Paolucci (CWR-W) 7-5, 1-6, 6-4 |
| C1 | Lily Cameron (DPUW) def. Kanthaphixay, Alaina (NCC) 6-0, 6-4 |
| C1 | Katherine Marks (ALLW) def. Monique Brual (DENW) 5-7, 6-4, 10-6 |
| C1 | Qingyue Li (WOOW) def. Barconi,Brianna (MILLIKIN) 6-1, 6-0 |
| C1 | Jayde Robinson (BW-W19) def. Gonzaga, Ysabel (ELMHURST) 6-0, 6-1 |
| C1 | Megan Collins (KENW) def. Viuhkonen, Lisa (NPU) 6-0, 6-1 |
| C1 | Maddy Winarski (KZOO-W) def. Tebatso Duba (LFC) 7-5, 6-1 |
| C1 | Magee, Michaela (AUGIE) def. Stechow, Claire (WITT) 6-0, 6-4 |
| C1 | Nicole Cardwell (CWR-W) def. Kyra Braun (TRINE-W) 6-2, 6-1 |
| C1 | Hougland, Hannah (NCC) def. Cami Jones (HC-W) 6-3, 6-1 |
| C1 | Meyers, Paige (WITT) def. Bettner,Morgan (MILLIKIN) 7-5, 7-6 (7-3) |
| C1 | Alex Cash (DENW) def. Sanjna Tripathy (WASHUW) 5-7, 6-3, 10-4 |
| C1 | Sydney Bergman (OTT) def. Ashtyn Morris (BW-W19) 6-2, 6-3 |
| C1 | Leeza Bodnar (LFC) def. Gaby DeLeon (ALLW) 6-2, 6-1 |
| C1 | Ashley Spirrison (TRINE-W) def. Schoeck, Lily (AUGIE) 6-3, 6-0 |
| C1 | Ishika Gupta (WOOW) def. Pasquini, Nina (ELMHURST) 6-1, 6-3 |
| C1 | Sarah Robertson (DENW) def. Viuhkonen, Linn (NPU) 6-2, 6-0 |
| 16 | Erika Oku (KENW) def. Savannah Bright (CWR-W) 6-2, 6-1 |
| 16 | Leah Newhof (HOPE) def. Hannah Johnston (WASHUW) 6-1, 6-2 |
| 16 | Amanda Bandrowski (HOPE) def. Zoe Park (WASHUW) 6-0, 6-2 |
| 16 | Semenov, Nicole (UCW) def. Ally Persky (WASHUW) 7-6 (7-5), 4-6, 6-2 |
| 16 | Daria Beshentseva (KENW) def. Trisha Sundaram (CWR-W) 6-1, 6-2 |
| 16 | Park, Lauren (UCW) def. Claire Hallock (HOPE) 2-6, 6-4, 6-2 |
| 16 | Sydney Jackson (HOPE) def. Leslie Ligier (WASHUW) 7-5, 6-2 |
| 16 | Xu, Catherine (UCW) def. Divya Sharma (WASHUW) 6-0, 6-0 |
| QF | Erika Oku (KENW) def. Leah Newhof (HOPE) 6-2, 7-6 (7-3) |
| QF | Semenov, Nicole (UCW) def. Amanda Bandrowski (HOPE) 7-5, 6-3 |
| QF | Park, Lauren (UCW) def. Daria Beshentseva (KENW) 4-6, 6-3, retired |
| QF | Xu, Catherine (UCW) def. Sydney Jackson (HOPE) 6-4, 6-2 |
| SF | Semenov, Nicole (UCW) def. Erika Oku (KENW) 6-0, retired |
| SF | Park, Lauren (UCW) def. Xu, Catherine (UCW) 6-3, 6-7 (0-7), 6-4 |
| F | Park, Lauren (UCW) def. Semenov, Nicole (UCW) 6-1, 6-0 |
| C2 | Lily Cameron (DPUW) def. Katherine Marks (ALLW) 6-1, 6-0 |
| C2 | Qingyue Li (WOOW) def. Jayde Robinson (BW-W19) 4-6, 6-2, 10-8 |
| C2 | Megan Collins (KENW) def. Maddy Winarski (KZOO-W) 6-3, 6-0 |
| C2 | Nicole Cardwell (CWR-W) def. Magee, Michaela (AUGIE) 6-0, 6-2 |
| C2 | Hougland, Hannah (NCC) def. Meyers, Paige (WITT) 6-0, 6-2 |
| C2 | Alex Cash (DENW) def. Sydney Bergman (OTT) 6-1, 7-5 |
| C2 | Ashley Spirrison (TRINE-W) def. Leeza Bodnar (LFC) 6-2, 6-0 |
| C2 | Sarah Robertson (DENW) def. Ishika Gupta (WOOW) 6-2, 6-2 |
| CQF | Lily Cameron (DPUW) def. Qingyue Li (WOOW) 6-0, 6-4 |
| CQF | Nicole Cardwell (CWR-W) def. Megan Collins (KENW) 7-6 (7-3), 6-3 |
| CQF | Alex Cash (DENW) def. Hougland, Hannah (NCC) 6-4, 6-4 |
| CQF | Sarah Robertson (DENW) def. Ashley Spirrison (TRINE-W) 6-3, 6-2 |
| CSF | Lily Cameron (DPUW) def. Nicole Cardwell (CWR-W) 7-5, 6-3 |
| CSF | Alex Cash (DENW) def. Sarah Robertson (DENW) 6-4, 6-4 |
| CF | Lily Cameron (DPUW) def. Alex Cash (DENW) 4-6, 6-1, 10-7 |
