| 1 | Grace Hederick (WASH) def. Madeline Brouwer (CALV) 7-6, 7-6 |
| 1 | Lena Rich (OBEW) def. Erin LaBarge (HOPE) 6-0, 7-6 |
| 1 | Mara Kaspers (KENW) def. Bella Russo (KZOO) 6-1, 6-1 |
| 1 | Katrin Gurvich (CWR-) def. Carol Vitellas (DENW) 6-2, 6-1 |
| 1 | Delaney Black (OBEW) def. Bailey Chorney (HOPE) 6-3, 6-2 |
| 1 | Kayla Pukys (KENW) def. Charlotte Purnode (DENW) 6-2, 7-6 |
| 1 | Jackie McDermott (OBEW) def. Molly Traverso (JCU-) 7-5, 6-2 |
| 1 | Meredith Breda (DPUW) def. Liz Pniewski (HOPE) 6-1, 6-0 |
| 1 | Badawi, Zeana (AUGI) def. Natalie Bourlas (JCU-) 2-6, 6-2, 10-3 |
| 1 | Celeste Woloshyn (WASH) def. Helen Pelak (KZOO) 6-2, 6-0 |
| 1 | Colbert, Morgan (IITW) def. Ola Latala (ONU) 6-2, 6-3 |
| 1 | Allyssa Krupp (JCU-) def. Maja Shaw Todorovic (OBEW) 7-5, 6-2 |
| 1 | Nicolette Bourlas (JCU-) def. Ashtyn Morris (BW-W) 6-2, 6-1 |
| 1 | Makayla Sutter (OTT) def. Jennifer Stroyne (JCU-) 6-3, 4-6, 10-3 |
| 1 | Megan Collins (KENW) def. Alex Oswalt (KZOO) 6-4, 6-4 |
| 1 | Madalyn Winarski (KZOO) def. Megan Davy (SMC) 6-3, 7-6 |
| 1 | Allison Watts (LFC) def. Sophie Allen (OTT) 7-6, 6-0 |
| 1 | Lauren Beier (CWR-) def. Grace Haigh (DPUW) 6-1, 6-4 |
| 1 | Skye Harwood (JCU-) def. Lilly Chamberlin (SMC) 6-1, 6-0 |
| 1 | Marriella Briones (OTT) def. Lucy Deal (DENW) 6-2, 7-5 |
| 1 | Leeza Bodnar (LFC) def. Libby Grant (DPUW) 6-2, 6-2 |
| 1 | Monica Davy (SMC) def. Gaudio, Chiara (ELMH) 6-3, 6-2 |
| 1 | Shannon Sertz (WOO) def. Kelsey Kopf (SMC) 7-6, 6-3 |
| 1 | Sophia Navas-Davis (OTT) def. Gonzaga, Ysabel (ELMH) 6-3, 6-4 |
| 1 | Sarah Casey (ALLW) def. Morgan Wagers (WOO) 6-7, 7-6, 10-5 |
| 1 | Sabine Worthoff (ONU) def. Wheeler, Kathryn (ELMH) 6-1, 6-0 |
| 1 | Imani Robinson (BW-W) def. Lauren Solano (ALBI) 3-6, 7-5, 10-5 |
| 1 | Bettner, Morgan (MILL) def. Olivia Conover (ALBI) 7-6, 6-4 |
| 1 | Lark Foote (CALV) def. Mackenzie Wills (ONU) 6-2, 6-2 |
| 1 | Kaleigh Cummins (ONU) def. Miharu Koh (ALLW) 6-7, 6-4, 10-4 |
| 1 | Julia Bachmann (KZOO) def. Juviel Vinluan (NPU) 6-0, 2-6, 10-8 |
| 1 | Grace Gould (LFC) def. Watts, Madison (WUWT) 6-2, 6-4 |
| 1 | Grace Hederick (WASH) def. Erin LaBarge (HOPE) 6-1, 6-3 |
| 1 | Katrin Gurvich (CWR-) def. Mara Kaspers (KENW) 6-4, 4-6, 11-9 |
| 1 | Lena Rich (OBEW) def. Bella Russo (KZOO) 6-2, 6-1 |
| 1 | Kayla Pukys (KENW) def. Delaney Black (OBEW) 7-5, 6-1 |
| 1 | Madeline Brouwer (CALV) def. Charlotte Purnode (DENW) 6-3, 6-4 |
| 1 | Bailey Chorney (HOPE) def. Ola Latala (ONU) 6-0, 6-2 |
| 1 | Celeste Woloshyn (WASH) def. Carol Vitellas (DENW) 6-3, 6-2 |
| 1 | Meredith Breda (DPUW) def. Molly Traverso (JCU-) 6-1, 6-1 |
| 1 | Jackie McDermott (OBEW) def. Badawi, Zeana (AUGI) 5-7, 7-5, 10-4 |
| 1 | Colbert, Morgan (IITW) def. Liz Pniewski (HOPE) 6-1, 6-4 |
| 1 | Helen Pelak (KZOO) def. Maja Shaw Todorovic (OBEW) 6-1, 6-3 |
| 1 | Natalie Bourlas (JCU-) def. Megan Collins (KENW) 6-1, 4-6, 10-7 |
| 1 | Nicolette Bourlas (JCU-) def. Alex Oswalt (KZOO) 2-6, 6-1, 10-6 |
| 1 | Allison Watts (LFC) def. Jennifer Stroyne (JCU-) 6-1, 6-0 |
| 1 | Makayla Sutter (OTT) def. Ashtyn Morris (BW-W) 6-7, 7-6, 10-7 |
| 1 | Sophie Allen (OTT) def. Madalyn Winarski (KZOO) 6-1, 6-3 |
| 1 | Allyssa Krupp (JCU-) def. Lauren Beier (CWR-) 6-3, 6-1 |
| 1 | Morgan Wagers (WOO) def. Grace Haigh (DPUW) 6-3, 6-4 |
| 1 | Skye Harwood (JCU-) def. Sabine Worthoff (ONU) 6-4, 6-4 |
| 1 | Marriella Briones (OTT) def. Lilly Chamberlin (SMC) 6-2, 4-6, 10-6 |
| 1 | Monica Davy (SMC) def. Shannon Sertz (WOO) 6-3, 6-2 |
| 1 | Leeza Bodnar (LFC) def. Kelsey Kopf (SMC) 6-4, 6-1 |
| 1 | Gaudio, Chiara (ELMH) def. Megan Davy (SMC) 6-3, 6-1 |
| 1 | Sophia Navas-Davis (OTT) def. Libby Grant (DPUW) 6-2, 6-3 |
| 1 | Imani Robinson (BW-W) def. Lucy Deal (DENW) 6-0, 6-3 |
| 1 | Lauren Solano (ALBI) def. Gonzaga, Ysabel (ELMH) 7-5, 6-7, 10-4 |
| 1 | Mackenzie Wills (ONU) def. Olivia Conover (ALBI) 6-2, 6-1 |
| 1 | Bettner, Morgan (MILL) def. Sarah Casey (ALLW) 7-6, 2-6, 10-4 |
| 1 | Lark Foote (CALV) def. Wheeler, Kathryn (ELMH) 6-2, 6-1 |
| 1 | Julia Bachmann (KZOO) def. Miharu Koh (ALLW) 6-2, 6-3 |
| 1 | Kaleigh Cummins (ONU) def. Grace Gould (LFC) 7-6, 6-3 |
| 1 | Juviel Vinluan (NPU) def. Watts, Madison (WUWT) 6-2, 2-6, 10-4 |
| 1 | Grace Hederick (WASH) def. Alyssa Seneviratne (DPUW) 6-3, 6-1 |
| 1 | Mira Amin (CWR-) def. Rainey Heck (OBEW) 6-4, 6-4 |
| 1 | Mayada Audeh (OBEW) def. L. Kelly (ONU) 6-3, 6-2 |
| 1 | Celeste Woloshyn (WASH) def. Ali Busch (DPUW) 6-1, 6-4 |
| 1 | Lena Rich (OBEW) def. J. Riddle (KZOO) 6-2, 6-3 |
| 1 | Delaney Black (OBEW) def. Bella Russo (KZOO) 6-3, 6-1 |
| 1 | Jackie McDermott (OBEW) def. Helen Pelak (KZOO) 6-2, 6-1 |
| 1 | Ola Latala (ONU) def. Maja Shaw Todorovic (OBEW) 6-2, 6-4 |
| 1 | Madalyn Winarski (KZOO) def. Elena Pienovitz (KENW) 6-1, 6-1 |
| 1 | Sabine Worthoff (ONU) def. Alex Oswalt (KZOO) 6-3, 3-6, 10-4 |
| 1 | Grace Haigh (DPUW) def. Katie Curry (ONU) 6-4, 3-6, 10-8 |
| 1 | Mackenzie Wills (ONU) def. Libby Grant (DPUW) 6-1, 6-3 |
| 1 | Kaleigh Cummins (ONU) def. Julia Bachmann (KZOO) 6-1, 6-0 |
