| 64 | Lauren O'Malley (JCU-W) def. Kate Lee (WASHUW) 6-1, 6-2 |
| 64 | Devin Gramley (DENW) def. Gaby DeLeon (ALLW) 7-6 (7-5), 7-5 |
| 64 | Handa, Claire (UCW) def. Natalie Rahrig (HOPE) 6-0, 6-1 |
| 64 | Rainie Heck (OBEW) def. Lynn Kelly (ONU) 6-0, 6-4 |
| 64 | Julie Lord (LFC) def. Maria Franco (KZOO-W) 6-3, 6-0 |
| 64 | Rudin, Alyssa (UCW) def. Ceylan Can (KENW) 6-3, 6-3 |
| 64 | Claire Hallock (HOPE) def. Lisa Chionis (WASHUW) 6-1, 6-1 |
| 64 | Nicole Cardwell (CWR-W) def. Ali Bush (DPUW) 2-6, 6-3, 6-1 |
| 64 | Diana Aboubakare (KENW) def. Madeline Hart (DENW) 6-1, 6-1 |
| 64 | Nina Cepeda (CWR-W) def. Travis, Adrienne (UCW) 6-1, 6-3 |
| 64 | Grace Deering (WASHUW) def. Christina Lombardi (SMC) by default |
| 64 | Behrle, Monica (WUWT) def. Jayde Robinson (BW-W) 6-0, 6-0 |
| 64 | Libby Jubas (WASHUW) def. Sarah Hughes (OBEW) 6-4, 1-6, 6-4 |
| 64 | Xu, Catherine (UCW) def. Taylor LaBarge (HOPE) 6-1, 6-2 |
| 64 | Madeleine Paolucci (CWR-W) def. Grace Winslow (KENW) 7-5, 6-0 |
| 64 | Timm, Annie (AUGIE) def. Amanda Showalter (DPUW) 7-5, 3-6, 6-4 |
| 64 | Gutierrez, Laura (UCW) def. ILANA Blackwood (KENW) 6-2, 6-3 |
| 64 | Ashley Lessen (WASHUW) def. Schaffer, Caitlyn (AUGIE) 1-6, 6-4, 7-6 (7-4) |
| 64 | Peyton Young (CWR-W) def. Claudia Olaes (OBEW) 3-6, 6-1, 6-2 |
| 64 | Amanda Bandrowski (HOPE) def. Laura Hanselman (KZOO-W) 5-7, 6-1, 6-2 |
| 64 | Ally Persky (WASHUW) def. Ellison, Daryn (UCW) 6-1, 6-2 |
| 64 | Halicka, Weronika (IITWOMEN) def. Madisyn Rini (JCU-W) 6-1, 6-1 |
| 64 | Mira Amin (CWR-W) def. Joy Li (WOO) 6-3, 6-0 |
| 64 | Lauren Hawley (DENW) def. Sydney Bergman (OTT) 6-0, 6-4 |
| 64 | Laurel Wanger (WASHUW) def. Quincey Glupker (HOPE) 7-5, 6-4 |
| 64 | Alyssa Seneviratne (DPUW) def. Mayada Audeh (OBEW) 1-6, 6-1, 6-1 |
| 64 | Annie Reiner (KENW) def. Leah Newhof (CALVIN) 4-6, 6-1, 6-3 |
| 64 | Antohi, Marjorie (UCW) def. Amanda Ress Liere (ALLW) 6-1 |
| 64 | Navarro, Estefania (UCW) def. Megan Hickey (DENW) 6-4, 6-1 |
| 64 | Erika Oku (KENW) def. Sanjna Tripathy (WASHUW) 6-0, 2-0, retired |
| 64 | Vilde Olvin (NPUW) def. Angell, Molly (MILLIKIN) 6-1, 6-1 |
| 64 | Nithya Kanagasegar (CWR-W) def. Ashley Ochsenhirt (ONU) 6-0, 6-2 |
| 32 | Lauren O'Malley (JCU-W) def. Devin Gramley (DENW) 6-0, 6-1 |
| 32 | Handa, Claire (UCW) def. Rainie Heck (OBEW) 6-0, 6-3 |
| 32 | Rudin, Alyssa (UCW) def. Julie Lord (LFC) 6-4, 6-1 |
| 32 | Claire Hallock (HOPE) def. Nicole Cardwell (CWR-W) 6-4, 6-3 |
| 32 | Diana Aboubakare (KENW) def. Nina Cepeda (CWR-W) 6-2, 6-2 |
| 32 | Grace Deering (WASHUW) def. Behrle, Monica (WUWT) 6-2, 6-2 |
| 32 | Libby Jubas (WASHUW) def. Xu, Catherine (UCW) 6-3, 6-4 |
| 32 | Madeleine Paolucci (CWR-W) def. Timm, Annie (AUGIE) 6-1, 6-0 |
| 32 | Ashley Lessen (WASHUW) def. Gutierrez, Laura (UCW) 6-3, 1-6, 6-2 |
| 32 | Amanda Bandrowski (HOPE) def. Peyton Young (CWR-W) 6-2, 6-1 |
| 32 | Halicka, Weronika (IITWOMEN) def. Ally Persky (WASHUW) 5-7, 6-4, 6-2 |
| 32 | Lauren Hawley (DENW) def. Mira Amin (CWR-W) 6-4, 7-5 |
| 32 | Laurel Wanger (WASHUW) def. Alyssa Seneviratne (DPUW) 7-5, 7-6 (12-10) |
| 32 | Antohi, Marjorie (UCW) def. Annie Reiner (KENW) 6-0, 6-0 |
| 32 | Erika Oku (KENW) def. Navarro, Estefania (UCW) 6-2, 7-5 |
| 32 | Nithya Kanagasegar (CWR-W) def. Vilde Olvin (NPUW) 6-0, 6-0 |
| 16 | Handa, Claire (UCW) def. Lauren O'Malley (JCU-W) 3-6, 6-4, 6-3 |
| 16 | Claire Hallock (HOPE) def. Rudin, Alyssa (UCW) 6-4, 6-4 |
| 16 | Grace Deering (WASHUW) def. Diana Aboubakare (KENW) 6-4, 3-6, 7-5 |
| 16 | Madeleine Paolucci (CWR-W) def. Libby Jubas (WASHUW) 0-6, 6-3, 6-4 |
| 16 | Ashley Lessen (WASHUW) def. Amanda Bandrowski (HOPE) 6-3, 2-6, 6-3 |
| 16 | Halicka, Weronika (IITWOMEN) def. Lauren Hawley (DENW) 6-2, 6-1 |
| 16 | Antohi, Marjorie (UCW) def. Laurel Wanger (WASHUW) 6-0, 6-2 |
| 16 | Nithya Kanagasegar (CWR-W) def. Erika Oku (KENW) 2-6, 7-6 (7-5), 6-2 |
| 8 | Handa, Claire (UCW) def. Claire Hallock (HOPE) by default |
| 8 | Grace Deering (WASHUW) def. Madeleine Paolucci (CWR-W) 6-0, 6-1 |
| 8 | Ashley Lessen (WASHUW) def. Halicka, Weronika (IITWOMEN) 6-4, 7-5 |
| 8 | Antohi, Marjorie (UCW) def. Nithya Kanagasegar (CWR-W) 6-0, 6-0 |
| SF | Handa, Claire (UCW) def. Grace Deering (WASHUW) 6-3, 6-3 |
| SF | Antohi, Marjorie (UCW) def. Ashley Lessen (WASHUW) 6-2, 7-6 (9-7) |
| F | Handa, Claire Antohi, Marjorie |
