| 128 | Nicole Pamphilis (DPUW) def. Katherine Hinman (MC) 6-2, 6-0 |
| 128 | Christi Valicenti (LFC) def. Hannah Vaselaney (ELM) 6-3, 6-1 |
| 128 | Alyssa Moreau (KENW) def. Nina Cepeda (CWRU-W) 6-4, 6-2 |
| 128 | Magee, Mollie (AUGIE) def. Kate Mader (HOPE) 6-1, 6-3 |
| 128 | Maria Franco (KZOO-W) def. Jackie McDermott (OBEW) 6-3, 2-6, 7-6 (3-0) |
| 128 | Maddie Lee (DPUW) def. Tamar Norville (IC) 6-0, 6-2 |
| 128 | Bequette, Paige (MILLIKIN) def. Alivia Livesay (WITW) 6-1, 6-2 |
| 128 | Warren, Courtney (UCW) def. Mikaela Pope (ALL) 6-3, 6-0 |
| 64 | Rebecca Ho (WASHUW) def. Nicole Pamphilis (DPUW) 6-3, 6-0 |
| 64 | Christi Valicenti (LFC) def. Olivia Hay (OBEW) 6-1, 6-0 |
| 64 | Kim, Rachel (UCW) def. Alyssa Moreau (KENW) 6-1, 6-2 |
| 64 | Lauren Hawley (DENW) def. Magee, Mollie (AUGIE) 6-3, 6-1 |
| 64 | Diana Aboubakare (KENW) def. Maria Franco (KZOO-W) 6-2, 6-0 |
| 64 | Maddie Lee (DPUW) def. Carlye Campagna (JCU-W) 6-0, 6-0 |
| 64 | Grace Deering (WASHUW) def. Bequette, Paige (MILLIKIN) 6-0, 6-0 |
| 64 | Meg Chiumento (DENW) def. Warren, Courtney (UCW) 6-1, 6-3 |
| 32 | Rebecca Ho (WASHUW) def. Christi Valicenti (LFC) 6-3, 6-1 |
| 32 | Kim, Rachel (UCW) def. Lauren Hawley (DENW) 6-2, 6-4 |
| 32 | Maddie Lee (DPUW) def. Diana Aboubakare (KENW) 1-6, 6-4, 6-4 |
| 32 | Meg Chiumento (DENW) def. Grace Deering (WASHUW) 6-1, 7-5 |
| 16 | Kim, Rachel (UCW) def. Rebecca Ho (WASHUW) 6-2, 6-1 |
| 16 | Meg Chiumento (DENW) def. Maddie Lee (DPUW) 6-3, 6-4 |
| QF | Kim, Rachel (UCW) def. Meg Chiumento (DENW) 7-6 (8-0), 7-6 (2-0) |
| 128 | Annie Reiner (KENW) def. Sarah Wilder (DPUW) 6-1, 6-4 |
| 128 | Katherine Devine (JCU-W) def. Taylor LaBarge (HOPE) 6-4, 1-6, 6-3 |
| 128 | Ava Kolman (ELM) def. Sloane Prince (ALL) 6-4, 4-6, 6-4 |
| 128 | Rayika Karachiwalla (WITW) def. Catherine Christian (WOO) by default |
| 128 | Emma Brezel (OBEW) def. MacDonald, Aileen (AUGIE) 6-3, 6-2 |
| 128 | Chen, Tiffany (UCW) def. Catherine Wingrove (HOPE) 6-2, 6-1 |
| 128 | Natalie Bourlas (JCU-W) def. Taryn Stromback (ONU) 4-6, 6-4, 6-2 |
| 128 | Lisa Chionis (WASHUW) def. Drone, Katie (NCC) 6-0, 6-1 |
| 64 | Taylor Hawkins (DENW) def. Annie Reiner (KENW) 6-4, 6-4 |
| 64 | Ellie Benzel (CALVIN) def. Katherine Devine (JCU-W) 6-4, 6-3 |
| 64 | Jessica Liu (CWRU-W) def. Ava Kolman (ELM) 6-3, 6-1 |
| 64 | Kaylan Griffith (WASHUW) def. Rayika Karachiwalla (WITW) 6-3, 6-1 |
| 64 | Nithya Kanagasegar (CWRU-W) def. Emma Brezel (OBEW) 6-1, 6-2 |
| 64 | Chen, Tiffany (UCW) def. Grace Dowling (LFC) 6-2, 6-4 |
| 64 | Adrienne D'Alewyn (OWU) def. Nicolette Bourlas (JCU-W) 6-3, 6-4 |
| 64 | Paige Cooley (PCWT) def. Lisa Chionis (WASHUW) 6-2, 6-1 |
| 32 | Taylor Hawkins (DENW) def. Ellie Benzel (CALVIN) 6-0, 6-1 |
| 32 | Jessica Liu (CWRU-W) def. Kaylan Griffith (WASHUW) 6-4, 6-3 |
| 32 | Nithya Kanagasegar (CWRU-W) def. Chen, Tiffany (UCW) 6-4, 3-6, 6-3 |
| 32 | Paige Cooley (PCWT) def. Adrienne D'Alewyn (OWU) 6-3, 6-0 |
| 16 | Jessica Liu (CWRU-W) def. Taylor Hawkins (DENW) 6-3, 6-3 |
| 16 | Nithya Kanagasegar (CWRU-W) def. Paige Cooley (PCWT) 7-5, 6-3 |
| QF | Nithya Kanagasegar (CWRU-W) def. Jessica Liu (CWRU-W) 6-1, 6-2 |
| 128 | Angell, Molly (MILLIKIN) def. Kathryn Ryan (IC) 6-1, 6-2 |
| 128 | Tang, Lucy (UCW) def. Brigitte Hodge (WASHUW) 6-1, 6-2 |
| 128 | Megan Galle (DPUW) def. Carli Milano (WITW) 6-4, 6-2 |
| 128 | Goggin, Dana (AUGIE) def. Billie Rodman (LFC) 1-6, 6-2, 6-1 |
| 128 | Claire Spoelhof (CALVIN) def. Heidi Mueller (ALL) 6-0, 4-6, 7-5 |
| 128 | Sara Torrence (DENW) def. Liddy Wilson (MC) 6-0, 6-1 |
| 128 | Ali Bush (DPUW) def. Brooke Butterworth (OWU) 6-1, 6-3 |
| 128 | Meghan Letizia (ONU) def. Chiara Gaudio (ELM) 6-1, 6-1 |
| 64 | Taylor Sweeney (CWRU-W) def. Kathryn Ryan (IC) 6-1, 6-0 |
| 64 | Tang, Lucy (UCW) def. Mayada Audeh (OBEW) 6-2, 6-0 |
| 64 | Megan Galle (DPUW) def. Katherine McCauley (KENW) 6-2, 6-2 |
| 64 | Nancy Benda (HOPE) def. Goggin, Dana (AUGIE) 6-1, 6-4 |
| 64 | Bynoe, Kaela (UCW) def. Claire Spoelhof (CALVIN) 6-0, 6-1 |
| 64 | Lauren O'Malley (JCU-W) def. Sara Torrence (DENW) 7-6 (6-0), 6-1 |
| 64 | Ali Bush (DPUW) def. Grace Winslow (KENW) 6-2, 6-1 |
| 64 | Sarah Woods (KZOO-W) def. Meghan Letizia (ONU) 6-1, 6-3 |
| 32 | Tang, Lucy (UCW) def. Taylor Sweeney (CWRU-W) 6-4, 6-3 |
| 32 | Nancy Benda (HOPE) def. Megan Galle (DPUW) 6-2, 6-1 |
| 32 | Bynoe, Kaela (UCW) def. Lauren O'Malley (JCU-W) 6-1, 6-4 |
| 32 | Sarah Woods (KZOO-W) def. Ali Bush (DPUW) 6-2, 6-3 |
| 16 | Nancy Benda (HOPE) def. Tang, Lucy (UCW) 7-5, 6-2 |
| 16 | Sarah Woods (KZOO-W) def. Bynoe, Kaela (UCW) 6-1, 7-6 (4-0) |
| 128 | Alyssa Senevirante (DPUW) def. Grizaffi, Miranda (NCC) 6-1, 7-5 |
| 128 | Kate Lee (WASHUW) def. Margaret Sweeney (KENW) 6-3, 7-6 (7-0) |
| 128 | Andrea Brush (ALL) def. Ariana Abayomi (OBEW) 6-4, 6-1, 6-3 |
| 128 | Stevanovich, Kat (UCW) def. Peyton Young (CWRU-W) 7-6 (2-0), 6-4 |
| 128 | Emma Alsup (DENW) def. Rachel Rettger (ELM) 6-1, 6-2 |
| 128 | Sara Zargham (CWRU-W) def. Meryl Reams (OWU) 6-1, 6-1 |
| 128 | Anna Garcia (HOPE) def. Schaffer, Cailtyn (AUGIE) 6-2, 6-4 |
| 128 | Colleen Morris (DPUW) def. Catherine Engel (JCU-W) 6-4, 6-3 |
| 64 | Alyssa Senevirante (DPUW) def. Kelly Daniel (HOPE) 1-6, by default |
| 64 | Kate Lee (WASHUW) def. Goggin, Lauren (AUGIE) 6-0, 7-5 |
| 64 | Andrea Brush (ALL) def. Jessica Bouma (CALVIN) 7-5, 6-2 |
| 64 | Stevanovich, Kat (UCW) def. Courtney Brown (DENW) 6-2, 1-6, 6-2 |
| 64 | Emma Alsup (DENW) def. Lynn Kelly (ONU) 6-2, 6-3 |
| 64 | Aly Coran (WASHUW) def. Sara Zargham (CWRU-W) 6-2, 6-3 |
| 64 | Anna Garcia (HOPE) def. Allison Watts (LFC) 6-1, 6-3 |
| 64 | Iranpour, Ariana (UCW) def. Colleen Morris (DPUW) 6-1, 6-4 |
| 32 | Kate Lee (WASHUW) def. Alyssa Senevirante (DPUW) 6-1, 7-5 |
| 32 | Stevanovich, Kat (UCW) def. Andrea Brush (ALL) 6-1, 6-1 |
| 32 | Aly Coran (WASHUW) def. Emma Alsup (DENW) 6-0, 6-1 |
| 32 | Iranpour, Ariana (UCW) def. Anna Garcia (HOPE) 6-2, 6-1 |
| 16 | Kate Lee (WASHUW) def. Stevanovich, Kat (UCW) 4-6, 6-2, 6-1 |
| 16 | Iranpour, Ariana (UCW) def. Aly Coran (WASHUW) 6-1, 6-1 |
| QF | Iranpour, Ariana (UCW) def. Kate Lee (WASHUW) 1-6, 6-3, 6-3 |
| SF | Nithya Kanagasegar (CWRU-W) def. Kim, Rachel (UCW) 6-4, 7-5 |
| SF | Iranpour, Ariana (UCW) def. Sarah Woods (KZOO-W) 6-4, 6-2 |
| F | Iranpour, Ariana (UCW) def. Nithya Kanagasegar (CWRU-W) 6-1, 6-2 |
| C | Brigitte Hodge (WASHUW) def. Mayada Audeh (OBEW) 6-4, 6-3 |
| C | Carli Milano (WITW) def. Katherine McCauley (KENW) 1-6, 6-3, 10-7 |
| C | Grace Winslow (KENW) def. Brooke Butterworth (OWU) 6-0, 6-1 |
| C | Grizaffi, Miranda (NCC) def. Kelly Daniel (HOPE) by default |
| C | Margaret Sweeney (KENW) def. Goggin, Lauren (AUGIE) 6-7 (0-5), 6-0, 10-9 |
| C | Ariana Abayomi (OBEW) def. Jessica Bouma (CALVIN) 6-1, 6-4 |
| C | Courtney Brown (DENW) def. Peyton Young (CWRU-W) 6-3, 6-0 |
| C | Lynn Kelly (ONU) def. Rachel Rettger (ELM) 6-2, 6-4 |
| C | Allison Watts (LFC) def. Schaffer, Cailtyn (AUGIE) 6-1, 6-2 |
| C | Brigitte Hodge (WASHUW) def. Angell, Molly (MILLIKIN) 6-1, 6-0 |
| C | Billie Rodman (LFC) def. Carli Milano (WITW) 6-2, 6-1 |
| C | Grace Winslow (KENW) def. Chiara Gaudio (ELM) by default |
| C | Margaret Sweeney (KENW) def. Grizaffi, Miranda (NCC) 6-1, 6-0 |
| C | Courtney Brown (DENW) def. Ariana Abayomi (OBEW) 6-2, 6-0 |
| C | Lynn Kelly (ONU) def. Meryl Reams (OWU) by default |
| C | Schaffer, Cailtyn (AUGIE) def. Catherine Engel (JCU-W) by default |
| C | Brigitte Hodge (WASHUW) def. Billie Rodman (LFC) by default |
| C | Courtney Brown (DENW) def. Margaret Sweeney (KENW) 8-3 |
| C | Schaffer, Cailtyn (AUGIE) def. Lynn Kelly (ONU) 8-4 |
| C | Brigitte Hodge (WASHUW) def. Grace Winslow (KENW) 8-0 |
| C | Courtney Brown (DENW) def. Schaffer, Cailtyn (AUGIE) 8-3 |
| C | Olivia Hay (OBEW) def. Hannah Vaselaney (ELM) 6-1, 6-3 |
| C | Carlye Campagna (JCU-W) def. Tamar Norville (IC) 6-2, 6-4 |
| C | Catherine Wingrove (HOPE) def. Grace Dowling (LFC) 4-6, 6-0, 10-3 |
| C | Nina Cepeda (CWRU-W) def. Kate Mader (HOPE) 6-0, 6-2 |
| C | Jackie McDermott (OBEW) def. Carlye Campagna (JCU-W) by default |
| C | Alivia Livesay (WITW) def. Mikaela Pope (ALL) by default |
| C | Sarah Wilder (DPUW) def. Taylor LaBarge (HOPE) 6-2, 6-1 |
| C | Catherine Wingrove (HOPE) def. MacDonald, Aileen (AUGIE) 6-3, 6-3 |
| C | Drone, Katie (NCC) def. Taryn Stromback (ONU) 6-2, 6-3 |
| C | Jackie McDermott (OBEW) def. Alivia Livesay (WITW) 6-0, 6-0 |
| C | Sarah Wilder (DPUW) def. Rayika Karachiwalla (WITW) by default |
| C | Catherine Wingrove (HOPE) def. Drone, Katie (NCC) 3-6, 6-3, 10-8 |
| C | Nina Cepeda (CWRU-W) def. Jackie McDermott (OBEW) by default |
| C | Sarah Wilder (DPUW) def. Catherine Wingrove (HOPE) by default |
| C | Nina Cepeda (CWRU-W) def. Sarah Wilder (DPUW) 8-2 |
| C | Brigitte Hodge (WASHUW) def. Courtney Brown (DENW) 8-4 |
| C | Brigitte Hodge (WASHUW) def. Nina Cepeda (CWRU-W) 8-6 |
