| 128 | Tang, Megan (UCW) def. Alyssa Senevirante (DPUW) 6-3, 6-3 |
| 128 | Grizaffi, Miranda (NCC) def. Chelsea Reeder (WITW) 6-2, 0-6, 6-3 |
| 128 | Rochell Issa (DENW) def. Adrien Leatherwood (OTT) 6-4, 7-5 |
| 128 | Surya Khadilkar (CWR-W) def. Amanda Susnak (HOPE) 6-0, 6-2 |
| 128 | Adrienne D'Alewyn (OWUW) def. Gabby Jensen (DPUW) 6-2, 6-0 |
| 128 | Rachel Mole (WOO) def. Lexi Chionis (LFC) 6-0, 6-4 |
| 128 | Emma Brezel (OBEW) def. Heather Fantry (KENW) 6-2, 6-1 |
| 128 | Eloise Germic (KZOO-W) def. Brittany Toney (ONUW) 6-2, 7-5 |
| 128 | Kaylan Griffith (WASHUW) def. Taylor Diffley (KENW) 7-6 (7-3), 6-3 |
| 128 | Nina Cepeda (CWR-W) def. Claire Spoelhof (CALVINW) 6-0, 6-1 |
| 128 | Carli Capestany (HOPE) def. Andrea Brush (ALLW) 6-1, 6-2 |
| 128 | Kaitlin Pickrel (DPUW) def. Heather Abzug (DENW) 6-3, 4-6, 6-2 |
| 128 | Nithya Kanagasegar (CWR-W) def. Erin Johnson (OBEW) 6-0, 6-4 |
| 128 | Jackie Kjolhede (SMC) def. Wilson, Randi (IWU) 6-2, 6-0 |
| 128 | Sabrina Dass (KZOO-W) def. Taryn Stromback (ONUW) 4-6, 6-1, 6-4 |
| 128 | Goggin, Dana (AUGIE) def. Schumann, Maggie (UCW) 6-1, 2-6, 6-3 |
| 128 | Rebecca Ho (WASHUW) def. Kelly Daniel (HOPE) 6-3, 6-0 |
| 128 | Emma Krummel (OTT) def. Parisa Bennett (WITW) 3-6, 6-0, 6-2 |
| 128 | Mayada Audeh (OBEW) def. Forkin, Kelsey (NCC) 6-1, 6-3 |
| 128 | Abigail Younger (KENW) def. Cheryl Zhang (KZOO-W) 6-3, 6-4 |
| 128 | Meg Chiumento (DENW) def. Mar, Gail (IWU) 6-0, 6-1 |
| 128 | McGillis, Kelsey (UCW) def. Christina Corbean (ONUW) 6-3, 6-3 |
| 128 | Sara Zargham (CWR-W) def. Carolyn Huerth (DPUW) 6-4, 6-3 |
| 128 | Kayle Sexton (SMC) def. Morgan Wagers (WOO) 6-0, 6-2 |
| 128 | Claire Marshall (DPUW) def. Ariana Abayomi (OBEW) 6-2, 6-4 |
| 128 | Erin Wong (WASHUW) def. Bequette, Paige (MILLIKIN) 6-0, 6-2 |
| 128 | Anna Garcia (HOPE) def. Diana Aboubakare (KENW) 6-3, 6-3 |
| 128 | Sloane Prince (ALLW) def. Rylie Markle (OTT) 7-6 (7-3), 6-1 |
| 128 | Chen, Tiffany (UCW) def. Kate Lee (WASHUW) 6-3, 6-1 |
| 128 | Kennedy Mulholland (CWR-W) def. Eleni Coleman (DENW) 6-1, 6-1 |
| 128 | Goggin, Lauren (AUGIE) def. Ellie Benzel (CALVINW) 6-1, 6-1 |
| 128 | Brooke Butterworth (OWUW) def. Siani Johnson (KZOO-W) 6-2, 6-3 |
| 128 | Kate Mader (HOPE) def. Liza Plakseychuk (DENW) 6-2, 6-1 |
| 128 | Hanna Newstadt (WASHUW) def. Magee, Mollie (AUGIE) 6-3, 6-3 |
| 128 | Andrea Fetters (SMC) def. Claudia Fenzi (WITW) 6-2, 6-1 |
| 128 | Taylor Mahr (DPUW) def. Alyssa Moreau (KENW) 6-2, 6-3 |
| 128 | Anna Treider (OBEW) def. Emily Harper (OTT) 7-5, 6-3 |
| 128 | Lee, Jasmine (UCW) def. Drone, Katie (NCC) 6-0, 6-0 |
| 128 | Lynn Kelly (ONUW) def. Kristen Ellefson (KZOO-W) 6-2, 6-2 |
| 128 | Marianne Bonanno (CWR-W) def. Linman, Josie (IWU) 6-0, 6-0 |
| 128 | Mikaela Pope (ALLW) def. Olivia Hay (OBEW) 3-6, 6-3, 6-4 |
| 128 | Grace Deering (WASHUW) def. Gretchen Wilder (DPUW) 6-0, 6-0 |
| 128 | Grace Dowling (LFC) def. Sabin, Aubrie (MILLIKIN) 6-1, 6-1 |
| 128 | Ramaswami, Sruthi (UCW) def. Taylor Sweeney (CWR-W) 6-1, 6-2 |
| 128 | Jenna Murray (KENW) def. Taylor Heasley (OWUW) 6-0, 6-0 |
| 128 | Maria Franco (KZOO-W) def. Alyx Lynham (OTT) 6-7 (4-7), 6-1, 6-2 |
| 128 | Maddie Boster (DENW) def. Megan Visser (CALVINW) 6-3, 6-0 |
| 128 | Nancy Benda (HOPE) def. Meghan Letizia (ONUW) 6-3, 6-0 |
| 128 | Sun Mo Koo (ALLW) def. Grace Winslow (KENW) 3-6, 6-2, 7-5 |
| 128 | Morgan Cohen (DPUW) def. Jessica Bouma (CALVINW) 7-6 (7-3), 6-0 |
| 128 | Iranpour, Ariana (UCW) def. Carrie Foura (KZOO-W) 6-0, 6-0 |
| 128 | Emily Holloway (DENW) def. Rose Chiumento (WOO) 6-2, 6-1 |
| 128 | Maya Hamaker (CWR-W) def. Sammi Kruger (OTT) 3-6, 6-3, 7-6 (10-8) |
| 128 | Lee, Stephanie (UCW) def. Jackie McDermott (OBEW) 6-2, 7-5 |
| 128 | Claire Cooper (HOPE) def. Whitney Lonnemann (OWUW) 6-2, 6-1 |
| 128 | Samantha Murphy (KENW) def. Natalie Rahrig (HOPE) 2-6, 7-5, 6-1 |
| 128 | Michelle Djohan (CWR-W) def. Hannah Meyer (DPUW) 6-1, 6-1 |
| 128 | Margaret Faller (SMC) def. Jade Brown (WITW) 6-0, 6-0 |
| 128 | Grace Porter (OBEW) def. Amber Lee (ONUW) 6-4, 2-6, 6-0 |
| 128 | Pence, Miranda (AUGIE) def. Olivia Underwood (DENW) 6-1, 7-6 (7-3) |
| 128 | Christi Valicenti (LFC) def. Olivia Bouchard (KZOO-W) 6-4, 6-7 (5-7), 6-4 |
| 128 | Warren, Courtney (UCW) def. Cross, Reynolds (NCC) 6-3, 6-1 |
| 128 | Jamie Silverberg (WASHUW) def. Dao, Brianna (IWU) 6-1, 6-0 |
| 64 | Tang, Megan (UCW) def. Chelsea Reeder (WITW) 6-0, 6-1 |
| 64 | Surya Khadilkar (CWR-W) def. Rochell Issa (DENW) 6-2, 6-7 (2-7), 6-1 |
| 64 | Adrienne D'Alewyn (OWUW) def. Rachel Mole (WOO) 4-6, 6-4, 7-6 (7-4) |
| 64 | Emma Brezel (OBEW) def. Eloise Germic (KZOO-W) 6-0, 6-0 |
| 64 | Kaylan Griffith (WASHUW) def. Nina Cepeda (CWR-W) 6-4, 6-3 |
| 64 | Kaitlin Pickrel (DPUW) def. Carli Capestany (HOPE) 6-4, 6-1 |
| 64 | Nithya Kanagasegar (CWR-W) def. Jackie Kjolhede (SMC) 6-0, 6-0 |
| 64 | Goggin, Dana (AUGIE) def. Sabrina Dass (KZOO-W) 7-5, 6-2 |
| 64 | Rebecca Ho (WASHUW) def. Emma Krummel (OTT) 6-1, 6-1 |
| 64 | Mayada Audeh (OBEW) def. Abigail Younger (KENW) 6-0, 6-0 |
| 64 | Meg Chiumento (DENW) def. McGillis, Kelsey (UCW) 6-2, 6-4 |
| 64 | Sara Zargham (CWR-W) def. Kayle Sexton (SMC) 6-1, 6-0 |
| 64 | Claire Marshall (DPUW) def. Erin Wong (WASHUW) 5-7, 6-1, 6-1 |
| 64 | Anna Garcia (HOPE) def. Sloane Prince (ALLW) 6-1, 6-1 |
| 64 | Chen, Tiffany (UCW) def. Kennedy Mulholland (CWR-W) 6-2, 6-0 |
| 64 | Goggin, Lauren (AUGIE) def. Brooke Butterworth (OWUW) 6-1, 6-3 |
| 64 | Hanna Newstadt (WASHUW) def. Kate Mader (HOPE) 7-5, 6-3 |
| 64 | Taylor Mahr (DPUW) def. Andrea Fetters (SMC) 6-2, 6-0 |
| 64 | Lee, Jasmine (UCW) def. Anna Treider (OBEW) 6-0, 6-0 |
| 64 | Marianne Bonanno (CWR-W) def. Lynn Kelly (ONUW) 6-3, 6-1 |
| 64 | Grace Deering (WASHUW) def. Mikaela Pope (ALLW) 6-1, 6-0 |
| 64 | Ramaswami, Sruthi (UCW) def. Grace Dowling (LFC) 6-0, 6-3 |
| 64 | Maria Franco (KZOO-W) def. Jenna Murray (KENW) 6-3, 1-6, 7-6 (7-3) |
| 64 | Nancy Benda (HOPE) def. Maddie Boster (DENW) 6-1, 6-1 |
| 64 | Sun Mo Koo (ALLW) def. Angell, Molly (MILLIKIN) 6-1, 6-1 |
| 64 | Iranpour, Ariana (UCW) def. Morgan Cohen (DPUW) 6-0, 6-0 |
| 64 | Emily Holloway (DENW) def. Maya Hamaker (CWR-W) 7-6 (7-2), 6-3 |
| 64 | Claire Cooper (HOPE) def. Lee, Stephanie (UCW) 6-3, 7-5 |
| 64 | Michelle Djohan (CWR-W) def. Samantha Murphy (KENW) 6-1, 6-3 |
| 64 | Grace Porter (OBEW) def. Margaret Faller (SMC) 6-2, 6-1 |
| 64 | Christi Valicenti (LFC) def. Pence, Miranda (AUGIE) 6-4, 7-6 (7-3) |
| 64 | Jamie Silverberg (WASHUW) def. Warren, Courtney (UCW) 7-6 (7-3), 6-3 |
| 32 | Tang, Megan (UCW) def. Surya Khadilkar (CWR-W) 6-0, 6-2 |
| 32 | Emma Brezel (OBEW) def. Adrienne D'Alewyn (OWUW) 6-2, 6-3 |
| 32 | Kaylan Griffith (WASHUW) def. Kaitlin Pickrel (DPUW) 6-3, 6-4 |
| 32 | Nithya Kanagasegar (CWR-W) def. Goggin, Dana (AUGIE) 6-1, 6-1 |
| 32 | Rebecca Ho (WASHUW) def. Mayada Audeh (OBEW) 6-0, 6-2 |
| 32 | Sara Zargham (CWR-W) def. Meg Chiumento (DENW) 6-1, 2-6, 7-5 |
| 32 | Claire Marshall (DPUW) def. Anna Garcia (HOPE) 6-1, 6-0 |
| 32 | Chen, Tiffany (UCW) def. Goggin, Lauren (AUGIE) 1-6, 6-2, 6-3 |
| 32 | Hanna Newstadt (WASHUW) def. Taylor Mahr (DPUW) 7-6 (7-5), 6-4 |
| 32 | Lee, Jasmine (UCW) def. Marianne Bonanno (CWR-W) 6-3, 6-2 |
| 32 | Grace Deering (WASHUW) def. Ramaswami, Sruthi (UCW) 6-1, 6-2 |
| 32 | Nancy Benda (HOPE) def. Maria Franco (KZOO-W) 6-0, 6-1 |
| 32 | Iranpour, Ariana (UCW) def. Sun Mo Koo (ALLW) 6-0, 6-0 |
| 32 | Claire Cooper (HOPE) def. Emily Holloway (DENW) 6-2, 6-2 |
| 32 | Grace Porter (OBEW) def. Michelle Djohan (CWR-W) 5-7, 7-5, 6-2 |
| 32 | Jamie Silverberg (WASHUW) def. Christi Valicenti (LFC) 6-0, 6-0 |
| 16 | Tang, Megan (UCW) def. Emma Brezel (OBEW) 6-3, 6-0 |
| 16 | Nithya Kanagasegar (CWR-W) def. Kaylan Griffith (WASHUW) 6-2, 6-0 |
| 16 | Rebecca Ho (WASHUW) def. Sara Zargham (CWR-W) 6-4, 6-3 |
| 16 | Chen, Tiffany (UCW) def. Claire Marshall (DPUW) 6-4, 4-6, 6-3 |
| 16 | Lee, Jasmine (UCW) def. Hanna Newstadt (WASHUW) 6-2, 6-1 |
| 16 | Grace Deering (WASHUW) def. Nancy Benda (HOPE) 0-6, 6-2, 6-2 |
| 16 | Iranpour, Ariana (UCW) def. Claire Cooper (HOPE) 6-3, 6-2 |
| 16 | Jamie Silverberg (WASHUW) def. Grace Porter (OBEW) 6-0, 6-3 |
| QF | Nithya Kanagasegar (CWR-W) def. Tang, Megan (UCW) 6-4, 7-5 |
| QF | Rebecca Ho (WASHUW) def. Chen, Tiffany (UCW) 6-1, 6-3 |
| QF | Lee, Jasmine (UCW) def. Grace Deering (WASHUW) 6-0, 6-3 |
| QF | Iranpour, Ariana (UCW) def. Jamie Silverberg (WASHUW) 6-4, 6-2 |
| SF | Rebecca Ho (WASHUW) def. Nithya Kanagasegar (CWR-W) 5-7, 6-4, 6-1 |
| SF | Iranpour, Ariana (UCW) def. Lee, Jasmine (UCW) 6-0, 6-1 |
| FI | Rebecca Ho (WASHUW) def. Lee, Jasmine (UCW) 7-5, 6-2 |
| C | Alyssa Senevirante (DPUW) def. Grizaffi, Miranda (NCC) 6-3, 6-1 |
| C | Amanda Susnak (HOPE) def. Adrien Leatherwood (OTT) 6-0, 6-2 |
| C | Gabby Jensen (DPUW) def. Lexi Chionis (LFC) 7-5, 4-6, 10-7 |
| C | Heather Fantry (KENW) def. Brittany Toney (ONUW) 6-1, 6-0 |
| C | Taylor Diffley (KENW) def. Claire Spoelhof (CALVINW) 6-1, 6-1 |
| C | Andrea Brush (ALLW) def. Heather Abzug (DENW) 6-0, 6-4 |
| C | Erin Johnson (OBEW) def. Wilson, Randi (IWU) 6-2, 6-1 |
| C | Kelly Daniel (HOPE) def. Parisa Bennett (WITW) 6-1, 6-0 |
| C | Forkin, Kelsey (NCC) def. Cheryl Zhang (KZOO-W) 6-2, 6-0 |
| C | Christina Corbean (ONUW) def. Mar, Gail (IWU) 6-1, 6-4 |
| C | Carolyn Huerth (DPUW) def. Morgan Wagers (WOO) 6-0, 6-0 |
| C | Ariana Abayomi (OBEW) def. Bequette, Paige (MILLIKIN) 6-0, 6-0 |
| C | Diana Aboubakare (KENW) def. Kate Lee (WASHUW) 6-3, 6-3 |
| C | Kate Lee (WASHUW) def. Eleni Coleman (DENW) 6-1, 6-2 |
| C | Ellie Benzel (CALVINW) def. Siani Johnson (KZOO-W) 6-3, 6-1 |
| C | Magee, Mollie (AUGIE) def. Alyssa Moreau (KENW) 6-2, 6-0 |
| C | Alyssa Moreau (KENW) def. Claudia Fenzi (WITW) 6-0, 6-0 |
| C | Drone, Katie (NCC) def. Emily Harper (OTT) 6-3, 6-0 |
| C | Kristen Ellefson (KZOO-W) def. Linman, Josie (IWU) 6-0, 6-2 |
| C | Gretchen Wilder (DPUW) def. Olivia Hay (OBEW) 6-0, 6-1 |
| C | Taylor Sweeney (CWR-W) def. Sabin, Aubrie (MILLIKIN) 6-0, 6-0 |
| C | Taylor Heasley (OWUW) def. Alyx Lynham (OTT) 6-2, 6-4 |
| C | Megan Visser (CALVINW) def. Meghan Letizia (ONUW) 1-6, 7-6 (7-4), 10-7 |
| C | Grace Winslow (KENW) def. Mindy Borovsky (WASHUW) walkover |
| C | Jessica Bouma (CALVINW) def. Carrie Foura (KZOO-W) 4-6, 6-2, 10-5 |
| C | Sammi Kruger (OTT) def. Rose Chiumento (WOO) 6-0, 6-0 |
| C | Jackie McDermott (OBEW) def. Whitney Lonnemann (OWUW) 6-0, 6-1 |
| C | Natalie Rahrig (HOPE) def. Hannah Meyer (DPUW) 6-0, 6-1 |
| C | Amber Lee (ONUW) def. Jade Brown (WITW) 6-0, 6-0 |
| C | Olivia Underwood (DENW) def. Olivia Bouchard (KZOO-W) 6-0, 6-1 |
| C | Dao, Brianna (IWU) def. Cross, Reynolds (NCC) 7-6 (7-4), 3-6, 10-8 |
| C | Alyssa Senevirante (DPUW) def. Amanda Susnak (HOPE) 6-1, 6-0 |
| C | Heather Fantry (KENW) def. Gabby Jensen (DPUW) 6-2, 6-1 |
| C | Taylor Diffley (KENW) def. Andrea Brush (ALLW) 6-1, 6-1 |
| C | Schumann, Maggie (UCW) def. Erin Johnson (OBEW) 2-6, 6-4, 10-3 |
| C | Kelly Daniel (HOPE) def. Forkin, Kelsey (NCC) 6-1, 6-0 |
| C | Carolyn Huerth (DPUW) def. Christina Corbean (ONUW) 6-1, 6-1 |
| C | Diana Aboubakare (KENW) def. Ariana Abayomi (OBEW) 6-1, 7-6 (7-4) |
| C | Kate Lee (WASHUW) def. Ellie Benzel (CALVINW) 6-2, 6-1 |
| C | Magee, Mollie (AUGIE) def. Alyssa Moreau (KENW) 6-3, 6-2 |
| C | Drone, Katie (NCC) def. Kristen Ellefson (KZOO-W) 6-1, 6-0 |
| C | Taylor Sweeney (CWR-W) def. Gretchen Wilder (DPUW) 6-3, 6-4 |
| C | Megan Visser (CALVINW) def. Taylor Heasley (OWUW) 6-2, 6-1 |
| C | Jessica Bouma (CALVINW) def. Grace Winslow (KENW) 6-2, 2-6, 10-8 |
| C | Sammi Kruger (OTT) def. Jackie McDermott (OBEW) 7-5, 6-1 |
| C | Amber Lee (ONUW) def. Natalie Rahrig (HOPE) 6-1, 6-3 |
| C | Olivia Underwood (DENW) def. Dao, Brianna (IWU) 6-2, 6-1 |
| C | Alyssa Senevirante (DPUW) def. Heather Fantry (KENW) 6-2, 6-2 |
| C | Taylor Diffley (KENW) def. Schumann, Maggie (UCW) 6-3, 6-0 |
| C | Carolyn Huerth (DPUW) def. Kelly Daniel (HOPE) 6-2, 6-4 |
| C | Diana Aboubakare (KENW) def. Kate Lee (WASHUW) 7-5, 6-1 |
| C | Magee, Mollie (AUGIE) def. Drone, Katie (NCC) 6-3, 6-2 |
| C | Taylor Sweeney (CWR-W) def. Megan Visser (CALVINW) 7-5, 6-2 |
| C | Sammi Kruger (OTT) def. Jessica Bouma (CALVINW) 6-0, 6-2 |
| C | Amber Lee (ONUW) def. Olivia Underwood (DENW) 6-4, 6-3 |
| CQ | Taylor Diffley (KENW) def. Alyssa Senevirante (DPUW) 6-3, 6-1 |
| CQ | Carolyn Huerth (DPUW) def. Diana Aboubakare (KENW) 7-6 (8-6), 6-0 |
| CQ | Magee, Mollie (AUGIE) def. Taylor Sweeney (CWR-W) 6-4, 6-2 |
| CQ | Amber Lee (ONUW) def. Sammi Kruger (OTT) 6-2, 6-1 |
| CS | Taylor Diffley (KENW) def. Carolyn Huerth (DPUW) 6-1, 6-4 |
| CS | Magee, Mollie (AUGIE) def. Amber Lee (ONUW) 6-4, 6-2 |
| CF | Taylor Diffley (KENW) def. Magee, Mollie (AUGIE) 6-3, 6-0 |
